Question by Ebony: What antifungal product should I use to prevent contamination in my cultures?
I have been trying to cultivate lactobacillus cultures, but they have been getting contaminated by fungus despite me closing the petriplates properly. I would appreciate some suggestions on what antifungal solution or powder to use and in what concentration. I require an answer rather urgently. Thanks!
Answer by Paul
Fungizone and Nystatin are the most common antifungals used for tissue culture but I have no idea whether they are compatible with bacterial culture. You might want to call the technical service folks at whatever company you purchase reagents from that sell these and ask them.
I have found that the most likely source of fungal contamination, especially if you keep seeing the same type of fungus, is the incubator itself. Especially if it is one that has a fan to circulate the air which just blows the spores everywhere. You might want to clean it thoroughly with bleach or other cleaner and leave a pan of it in overnight to let the vapors get into the crevices (not while you are culturing anything).
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